SOP of Complete Blood Count

Table of Contents

1. Purpose

2. Safety Requirements

3. Associated Documents

4. Notes

5. Quality Control

6. Equipment

7. Supplies

8. Procedures

1.Purpose


1.1 : The purpose of complete blood count which known as CBC is used to evaluate the compositions and concentration of blood. Complete blood count provides 22 measurements related to blood cells, including all information related to white blood cell count, neutrophils, lymphocytes, monocytes, eosinophils, basophils, red blood cell count, hemoglobin concentration, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and concentration, red cell distribution width, platelet count, mean platelet volume, platelet distribution width, and plateletcrit.

2. Safety Requirements


2.1 : General laboratory procedures should be followed, which include: no eating, no chewing gum, no drinking, and no applying of cosmetics in the work area. Laboratory coats and gloves must be worn at all times in the work area, unless the protocol specifically describes the appropriate attire for the procedure.

3. Associated Documents


3.1 : Abbott Cell-Dyn 3700

4. Notes


4.1 : Wear protective gloves while collecting blood sample to avoid contacting with pathological microbes.

4.2 : Sufficient amount of blood is required (~260μl) to analyze up to 22 parameters.

4.3 : Deliver the blood sample within 4 hours after collection if possible and seal the sample at low temperature (4℃) and deliver to TMC as soon as possible.

4.4 : Do not centrifuge the blood sample.

4.5 : Do not adulterate the blood sample.

4.6 : Do not expose the blood sample to sunlight directly.

4.7 : Do not provide blood sample with clots.

4.8 : Blood sample is not allowed to use heparin as anticoagulant.

5. Quality Control


5.1 : Three Cell-DYN® 26 Plus Control which are Low, Normal and High respectively are used to ensure the accuracy and precision of Abbott Cell-Dyn 3700.

6. Equipment


6.1 : Abbott Cell-Dyn 3700

6.2 : Cell-DYN® 26 Plus Control

6.3 : Cell-DYN® Enzymatic Cleaner Concentrate

7. Supplies


7.1 : Whole blood (~260μl)

7.2
: Glove

7.3
: Paper towel

7.4
: Wiper

7.5
: Microtainer with K2E (tube with K2EDTA)

7.6
: 70% alcohol

7.7
: 5 mm lancet

7.8
: Age 8 weeks mice with both sex

8. Procedures


8.1 : Collect about 260μl of whole blood gently from check by pouching the lancet

8.2 : Transfer whole blood to 500μl K2E coated microtainer with extreme care.

8.3 : Shake whole blood sample softly and make sure the blood cells are well suspended to avoid blood clotting

8.4 : Allow the three Cell-DYN® 26 Plus Controls come to room temperature

8.5 : Turn on Abbott Cell-Dyn 3700

8.6 : Shake the three controls softly and make sure the blood cells are well suspended

8.7 : Run the three controls

8.8 : Check the setting

8.9 : Set the ID number

8.10 : Prepare test sample to come to room temperature

8.11 : Shake test sample softly and allow the blood cells are well suspended

8.12: Aspire the blood sample

8.13 : Result obtained

8.14 : Wash Abbott Cell-Dyn 3700 by using Cell-DYN® Enzymatic Cleaner Concentrate

8.15 : Turn off Abbott Cell-Dyn 3700